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KMID : 0624620090420050281
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BMB Reports
2009 Volume.42 No. 5 p.281 ~ p.285
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Antifungal activity of Saccharomyces cerevisiae peroxisomal 3-ketoacyl-CoA thiolase
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Lee Jung-Ro
Kim Sun-Young
Chae Ho-Byoung
Jung Ji-Hyun
Lee Sang-Yeol
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Abstract
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Peroxisomes play an important role in cellular defense systems and generate secondary messengers for cellular communication. Saccharomyces cerevisiae containing oleate-induced peroxisomes were subjected to buffer-soluble extraction and two chromatographic procedures, and a protein with antifungal activity was isolated. The results of MALDI-TOF analysis identified the isolated protein as peroxisomal 3-ketoacyl-CoA thiolase (ScFox3). Purified yeast ScFox3 exhibited thiolase activity that catalyzed the thiolytic cleavage of 3-ketoacyl-CoA to acetyl-CoA and acyl-CoA. ScFox3 protein inhibited various pathogenic fungal strains, with the exception of Aspergillus flavus. Using ScFox3-GFP and PTS2 signal-truncated ScFox3M-GFP, we showed that only ScFox3-GFP, with an intact PTS2 peroxisome signal sequence, was able to translocate into peroxisomes. Yeast ScFox3 is a natural antifungal agent found in peroxisomes.
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KEYWORD
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Antifungal activity, Hemolytic activity, Saccharomyces cerevisiae, ScFox3, Thiolase activity
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